Abstract: Clarifying the role of government intervention in the technological development of regional strategic emerging industries is of great significance to the high-quality development of China's economy and innovation-driven development. For the relationship between government intervention and the evolution of urban technological paths, this paper argues that regional knowledge base and innovation origin ability play an important role in it, and examines it from both theoretical and empirical aspects.Theoretically, by constructing a mediated moderation model, it demonstrates that the government intervention effect on the technological paths is affected by the moderation mechanism of the local knowledge base, and innovation origin ability plays a partly mediating role. This model expands the analytical framework for the impact of government intervention on technological paths. Empirically, based on the invention patent data of strategic emerging industries in 139 cities in China from 2010 to 2020, it tests the effect and internal mechanism of government intervention on urban technological development paths and estimates the size of the direct effect and the mediation effect. And for the first time, referring to the innovation radiation and science and technology governance connotation of the innovation origin ability, this research improves its measuring method, and uses the centrality of the patent transfer network to describe the innovation origin ability to highlight its innovation radiation effect. The study finds that government intervention promotes the development of technological paths of strategic emerging industries in cities, and the city's knowledge base plays a positive moderating role; compared with the stage of technological path-creation without comparative advantage, government intervention can have a more significant impact on the stage of path maintenance that already has a comparative advantage; innovation origin ability plays a partly mediating role between government intervention and technological paths, and the mediating effect mainly depends on the first half of the process, and part of the moderating effect of knowledge base is achieved through the innovation origin ability. In terms of policy, putting forward a new model in which government intervention needs to be closely integrated with the regional knowledge base and strengthen the construction of regional innovation origin ability, promoting the organic combination of government intervention and market mechanism in the evolution of technological paths. This paper has important reference significance for the government to use the regional knowledge base and enhance the innovation origin ability to promote the evolution of technological paths, and to promote the high-quality development of China's strategic emerging industries.