低聚原花青素对糖尿病肾病大鼠足细胞上皮-间质转化的抑制作用

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摘要目的探讨低聚原花青素(oligoprocyanidins, OPC)对糖尿病肾病(diabetic kidney disease,DKD)大鼠足细胞上皮-间质转化(Epithelial-to-Mesenchymal transition, EMT)的影响。方法选择雄性SD大鼠20只,用链脲佐菌素、高脂高糖饮食制造DKD模型。成模后随机分为模型组和OPC组(常规饲养基础上加喂OPC),另取10只雄性SD大鼠作为对照组。8周后比较各组大鼠的肾功能及组织形态学变化。Western blot分析EMT相关蛋白的水平。结果 OPC能明显降低糖尿病肾病大鼠血糖、血肌酐、尿素氮和尿蛋白水平(P<0.05),减轻DKD大鼠的肾组织形态学改变。电镜下OPC组大鼠足细胞部分融合,出现自噬泡。OPC上调DKD大鼠足细胞的E-cadherin蛋白的表达,下调Vimentin、Twist1蛋白的表达。结论 OPC可诱导DKD大鼠足细胞自噬,减轻足细胞EMT,为DKD的进一步研究提供了基础。

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关键词 ：肾小球足细胞, 上皮-间质转化, 原花青素, 自噬

Abstract：Objective To investigate the effect of oligoprocyanidins(OPC) on epithelial-to-mesenchymal transition (EMT) of podocytes in rats with diabetic kidney disease (DKD). Methods Thirty male SD rats were randomly divided into the control group, model group and OPC group [500 mg/(kg·d), 8 weeks]. The renal function of different groups was compared. The histological changes were observed via light and electron microscopy. The levels of EMT-related proteins were analyzed by Western blot. Results OPC could significantly reduce the levels of blood glucose, serum creatinine, urea nitrogen and urinary protein in DKD rats (P<0.05). Under an electron microscope, podocytes in the OPC group partially fused and autophagic vesicles appeared. OPC up-regulated the expression of E-cadherin but down-regulated the expression of vimentin and Twist1 in podocytes of DKD rats. Conclusions OPC can induce podocyte autophagy and reduce podocyte EMT in DKD rats.

Key words： glomerular podocytes epithelial-to-mesenchymal transition oligoprocyanidins autophagy

收稿日期: 2021-02-10

ZTFLH:

R587

作者简介: 吕晓楠,硕士,主治医师。

引用本文:

吕晓楠, 宗春辉, 董铮. 低聚原花青素对糖尿病肾病大鼠足细胞上皮-间质转化的抑制作用[J]. 武警医学, 2021, 32(7): 616-619. LV Xiaonan, ZONG Chunhui, DONG Zheng. Inhibitory effect of oligoprocyanidins on podocyte epithelial-to-mesenchymal transition in rats with diabetic kidney disease. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(7): 616-619.

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http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2021/V32/I7/616

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