Abstract: To evaluate objectively the Innovation Ecosystem Cooperativity (IEC) is conductive to promoting regional sustained and healthy development of hi - tech industries. Through using innovation ecosystem theory, Lotka-Volterra model, cluster analysis method, this paper constructs a set of relatively systematic classified evaluation system of IEC in regard to regional hi – tech industries. Based on the statistical data of China’ s hi - tech industries’ statistical yearbook (1997 - 2014), from the perspective of Production and Management (PM), R&D activities (RD) and investment in Fixed Assets (FA), classified evaluation results and optimizing countermeasures for China’ s 31 provinces and cities’ IEC in regard to regional hi - tech industries are provided in this paper. The study shows that the IEC of China’s hi - tech industry is generally quite good. However, great disparities exist for different rank of PM and equilibrium state when comparing hi - tech industries in different types of provinces and cities. To improve the efficiency of FA and RD, and to promote the coordination of RD and FA, are main path to improve IEC of hi – tech industries in the provinces and cities with some disequilibrium. The results of this study provide some practical enlightenments for different types of provinces and cities in improving IEC of hi - tech industries.