Abstract: Technology transfer practices are a series of actions taken by technology transfer agencies to promote technology transfer.Based on the multi-case study of five "Double-Firstclass" universities, this paper identifies the specific forms of technology transfer practices and the main barriersto technology transfer in universities and explains the mechanism of technology transfer practicesdiminishingtechnology transfer barriers. The findings are as follows. (1) Technology transfer practices include internal management simplification, external network building, boundary spanning and culture management, among which internal management simplification is a unique and important practice in China. (2) Internal management simplification can reduce management barriers by reducing complexity and uncertainty. External network building and boundary spanningalleviate resource barriers by acquiring external resources and improving the efficiency of resource allocation. Boundary spanning and culture management overcome cultural barriers through information translation and personnel empowerment. In order to promote technology transfer effectiveness, universities should fully integrate and optimize the technology transfer organization system and attach more importance to diverse technology transfer practices.