Abstract: At present, the deficiency of research on entrepreneurial ecosystem is more reflected in the lack of research on cross-integration between different levels of the system, which easily leads to the deviation between the behavior of new enterprises and the development direction of the system. Only when the new enterprise in different system niche is integrated into the surrounding entrepreneurial ecosystem can it make itself and the system become a whole, so as to obtain system benefits and create achievements. This is also in line with the principle of common interest. However, research from the perspective of the integration of enterprises and systems is very rare. Therefore, this research emphasizes that new enterprises need to adopt the strategic orientation of “integration and isomorphism” with the system, and then puts forward a new concept of entrepreneurial ecological orientation. Besides, since the integration of opportunity and resource has become a key behavior for new ventures to obtain entrepreneurial performance, this research will further test the relationship among entrepreneurship ecological orientation, integration of opportunity and resource and entrepreneurial performance based on the standard research paradigm of “guide-behavior-performance”. The research conclusions can not only accurately grasp the formation process of new ventures’ strategic orientation under the entrepreneurial ecosystem, but also comprehensively reveal and summarize the entrepreneurial behavior rules of new ventures under the entrepreneurial ecosystem, which plays an important role in promoting entrepreneurial theory research.