Abstract: Abstract： Based on the knowledge chain as the research object， this paper analyzes the forming mechanism of knowledge chain synergistic effect from three aspects of subject coordination， coordination and knowledge collaboration， and put forward the relational research hypothesizes. Using the data of member organizations in the knowledge chain and the structural equation model， the research hypothesizes can be empirically tested. The conclusion shows that： the subject coordination， coordination mechanism and knowledge collaboration have direct impact on the knowledge chain synergistic effect. In addition， the coordination mechanism has a positive effect to subject coordination and knowledge collaboration in the organizations of knowledge chain. However， subject coordination has no significant effect for the subject coordination.