Abstract: It is not to interpret the innovation and development of metropolitan innovation system very well through the traditional perspective. Some scholars have paid attention to study the metropolitan innovation system from knowledge ecology and produced some research results. According to the current literatures, the paper abstracts the themes of two research felids in which are the relationships between knowledge ecology and metropolitan innovation system and the study of metropolitan innovation system based on knowledge ecology, then analyses and reviews the existing literatures by using the thematic analysis method. The results show that knowledge ecology impacts on innovation activities of metropolitan innovation organizations. And there are the relationships that those are interconnection of factors and synchronization of mechanisms within both. We can to expand the research of innovation structure and innovation mechanism of metropolitan innovation system based on knowledge ecology. Finally, the paper points out some limits of the existing studies and suggests the future research topics in the matters of theory expansions, empirical analyses and research perspectives.