Abstract: Based on related literatures of university-industry-government triple helix and entrepreneurial researches, in this paper we disclose the black box of internal operation in the triple-helix system. From the three characteristics dimensions of the triple helix system, namely, collaboration, network relationship and complementary synergy, and with regional entrepreneurship environment as the intermediary variable, this paper empirically examine the influence of the triple helix system of university-industry-government collaboration on the level of regional entrepreneurship. The results show that the collaboration, network relations, and complementary synergy have significantly positive influences on the level of entrepreneurship, and so does the regional entrepreneurial environment. The group analysis based on regional classification shows that, in the regions with higher level of economic development, the factors of collaboration and network relationship have significant positive influence on the level of regional entrepreneurship, while in the regions with the ordinary level of economic development, it is the factor of complementary synergy among the triple helix system that causes the significant influence on the level of regional entrepreneurship in China.