Abstract: The multiple interaction and resource cooperation among the university, government and industry can facilitate the development of the university entrepreneurial talent training program. Our research, based on the triple helix theory, intends to construct a conceptual model in which four factors influence one another like government – industry – university participation, relevant entrepreneurial competence of teachers and students, entrepreneurial talent training performance, and government – industry – university cooperation. Supposing the relevant entrepreneurial competence of teachers and students as the meditation variable, and the government – industry – university cooperation as the moderation variable, we testify our research through extensive questionnaires and data analysis and come to the main conclusions as below. First of all, the government – industry – university participation reveals a distinctive positive effect on the entrepreneurial talent training performance. Next, the relevant entrepreneurial competence of teachers and students shows a partial meditation between the government – industry – university participation and the entrepreneurial talent training performance. Thirdly, the government – industry – university cooperation has a notably positive moderation on the government or the university participation and the entrepreneurial talent training performance. It is expected the theoretical analysis and empirical methodology in this research can broaden the triple helix theory and also deepen the scope of the university entrepreneurial talent training program.