Abstract: With the increasing investment by government and universities, the connotation of entrepreneurship education in Colleges and universities is not only the cultivation of entrepreneurial attitude and consciousness, but also the knowledge and skills training in enterprise operations, financing, etc. Under the background of the deepening role of the entrepreneurship education, this research intends to explore whether the entrepreneurial education will affect the entrepreneurial performance and the relationship between entrepreneurial education, entrepreneurial experience and new venture performance. This paper reviews the theory of relationship between entrepreneurship education, enterprise performance and entrepreneurial experience, collecting data from Zhejiang University Science and Technology Park by questionnaire method. Finally, based on statistical analysis method, this paper tests the relationship between entrepreneurship education, entrepreneurial experience and entrepreneurial performance. The results show that the formal entrepreneurship education has a significant positive effect on the performance of the new venture enterprise, and the informal entrepreneurship education has no significant effect on the performance of the new venture enterprise. Entrepreneurial experience does not moderating the relationship between entrepreneurial education and entrepreneurial performance.