Abstract: Entrepreneurial intention is the best indicator for predicting entrepreneurial behaviors. The researchers with different academic backgrounds have different perspectives on entrepreneurial intention. Based on an integrated perspective of personal factor and opportunity factor, this paper discusses the influence and mechanism among perceived innovation of opportunity, chronically regulatory focus and entrepreneurial intention. The results show that: (1) perceived innovation of opportunities is positively correlated with entrepreneurial intention; (2) the chronical promotion focus strengthens the positive relation between perceived innovation of opportunities and entrepreneurial intention, which means in terms of the strong promotion focus, the higher newness of opportunity will be more likely to stimulate entrepreneurial intention; (3) the chronical prevention focus weakens the positive relation between perception on innovation of opportunities and entrepreneurial intention. This paper further enriches regulatory focus theory and the relevant researches about entrepreneurial intention, and it helps to explore the effective ways to transfer opportunities for innovation into entrepreneurial intention. What’s more, it plays a guiding role in the formulation of entrepreneurship policy.