Abstract: The international entrepreneurial experience of entrepreneurs will not only affect the knowledge acquisition of international market, but also influence the choice of innovation strategy. Therefore, exploring the effect of international entrepreneurial experience and market knowledge accumulation on ambidexterity innovation of born globals is a complex and interesting question. This study uses partial least squares (PLS) modeling and SmartPLS4 software in order to analyze the data of 196 survey questionnaires. The results show that the international innovation experience and market knowledge accumulation is the driving mechanism of born globals to carry out the strategy of innovation; The international entrepreneurial experience does not directly affect the exploratory innovation of born globals, but it presents a positive correlation to its exploitative innovation. The market knowledge accumulation will positively influence the exploratory innovation and exploitative innovation of born globals. There is a positive correlation between the exploitative innovation of born globals and the exploratory innovation. The scale of born globals is positively correlated with the market knowledge accumulation; Market uncertainty is positively correlated with the exploratory innovation and exploitative innovation of born globals. The research conclusion provides useful experience for the healthy development of China's born globals.