Abstract: Building the innovation ecosystem and playing a symbiotic role is the preferred strategy for promoting the development of regional sci-tech innovation. Combined with relevant data from 30 provinces in China from 2007 to 2015, the symbiosis level of China's regional innovation ecosystem using the symbiotic measurement model is measured, and the relationship between regional innovation ecosystem symbiosis and sci-tech innovation is empirically analyzed by establishing the static and dynamic panel data model. The results show that: the symbiosis degree of regional innovation ecosystem is generally characterized by "U", the eastern region is much higher than the central and western regions, the regional differences are large and the decline is still weak. In the short term, innovation ecosystem symbiosis has a relatively weak role in promoting sci-tech innovation, and the effect of symbiosis unit is not significant, in the long term, the positive driving effect of innovation ecosystem symbiosis is obviously amplified, and the symbiosis unit has a negative inhibitory effect on sci-tech innovation. The accumulative effects of sci-tech innovation are obvious, the symbiosis matrix, symbiosis platform, and symbiosis environment always have positive driving effects on sci-tech innovation, and the positive driving effects of symbiosis network is not obvious.