Abstract: The May 4th New Culture Movement opened a new era for the development of scientific culture in China, and its great achievements were attributed to its appropriate strategies of the dissemination of scientific culture. These strategies includes maintaining the harmony between utilitarianism and exhaustiveness, creating the discourse system of scientific culture, taking youth as the key audience for the dissemination of scientific culture, striving to build a phenomenal platform for propaganda, synthetically innovating based on the accommodation of Chinese culture and Western culture. Summarizing the experience and lessons of the dissemination of scientific culture during the May 4th New Culture Movement is of great significance to the current construction of scientific culture in china.