Abstract: Based on the B-Z reaction model, this paper constructed a three-dimensional Logistic evolution model of knowledge sharing, knowledge integration and knowledge creation in the process of knowledge flow of enterprise collaborative original innovation with reciprocal culture as the control variable. Using 216 valid questionnaires and Matlab software, this paper simulated the evolution trend of knowledge flow of enterprise collaborative original innovation under the circumstances of three different initial states when the intensity of reciprocal culture varied. The results show that in the case of the same intensity of reciprocal culture, when knowledge sharing, knowledge integration and knowledge creation are all focused on at the early stage of knowledge flow, knowledge creation improves faster than that when only knowledge sharing or knowledge sharing and knowledge integration are focused on. Reciprocal culture can promote the evolution of knowledge flow of enterprise collaborative original innovation, and strong reciprocal culture can shorten the cycle of the evolution of knowledge flow. This study provides the basis for enterprises to implement collaborative original innovation mode and improve original innovation capability.