Abstract: As knowledge producers and innovators, colleges should serve the strategy of Rural Revitalization which is a glorious mission given by the times. However, due to the constraints of knowledge attributes, absorptive capacity, acceptance willingness, institutional environment and so on, the knowledge spillover of rural rejuvenation in colleges has some problems, such as the mismatch of knowledge demand, the process and the way of spillover are not smooth. Through in-depth interviews and questionnaires, this paper investigated the status quo of knowledge spillover performance and its influencing factors in rural rejuvenation of college services. Structural equation modeling method is used to verify the hypothesis from three aspects of knowledge source, recipient and related scenarios, revealing the influencing factors and mechanism of knowledge spillover performance of rural revitalization in colleges. The results showed that:among the factors affecting the performance of knowledge spillover from colleges to rural areas, "innovation ability" and "spillover willingness" of knowledge source have significant positive influences on both "tangible performance" and "potential performance”. The "task oriented trust" and "policy environment” of associated scenarios have positive influences on both "tangible performance" and "potential performance”, while the "knowledge distance" and "cultural distance" have negative influences on both performances. Meanwhile, The "absorption capability", "cooperation expectation", "cooperation experience” and "acceptance willingness" of receivers have positive influences on improving spillover performances. This study provides policy recommendations for promoting the strategies of colleges serving rural revitalization.