Abstract: In order to explore the impact of the Internet on the knowledge increment in industry-university-research collaborative innovation.From the perspective of knowledge ecology, this paper constructs an internet-based collaborative innovation knowledge ecosystem model, and explains the process of knowledge appreciation by using ecological mechanism.On this basis, "huawei & tsinghua" collaborative innovation case was cited to build a dynamic model of knowledge appreciation system based on real data, and Vensim PLE software was used for simulation.The research shows that the Internet can improve the knowledge innovation environment of the knowledge population, enhance the knowledge transfer ability and willingness of the knowledge population, and provide knowledge services for the knowledge population to promote the knowledge increment in the collaborative innovation of the industry, the university and the research institute.Finally, some Suggestions are put forward on how to give full play to the role of Internet in promoting knowledge appreciation.