Abstract: Abstract: Start-ups have to face the matching problem between external resources context and their resources-focused action during development. Through the case study, we investigate the evolutionary path and mechanism of resource-focused action in start-up business. The result shows:(1) the resource-focused action of start-up business goes through resource bricolage, resource configutation to resource orchestration during development; (2) entrepreneurship, external opportunity and orgnizational capability are the main influence factors; (3) the revolution of entrepreneurship, external opportunity and orgnizational capability promote the revolution of resource-focused action. This research develops the basic awareness of resource-focused action and puts forward the evolutionary mechanism of resource-focused action, which develops RBT and and guides the resources-focused action of start-ups.