Abstract: Through the depth interview and questionnaire survey, surveying on the farmer cooperatives, local governments and the family farm of Jiangsu province about the current situation of knowledge spillover, and deal with of 482 samples’ data by SPSS20.0 and AMOSS17.0. The results show that knowledge distance and cultural distance between University and countryside have significant negative effects on the absorption ability and knowledge spillover effects, knowledge absorptive capacity of countryside plays an partial intermediary role between distance and knowledge spillover effects. This study provides a theoretical support for revealing the mechanism of knowledge spillover between the University and countryside.