Abstract: The cross-industrial upgrading of emerging market economies is becoming the focus of business and academia. Through literature review, this paper constructs an integrated analytical framework for cross-industry upgrading, strategic transformation and organizational response, and analyzes the process and mechanism of cross-industrial strategic transformation as well as the strategic response behavior of organizational structure. In the case of Shenzhen DaShi, this paper reveals the interactive process of "Chandler's Structure follows strategy" and its impact on enterprise competitiveness and upgrade performance. It is found that dynamically adjusting the organizational structure to adapt to the complexity of management decisions brought by cross-industry and cross-regional transformation strategies is the key to the successful transformation of DaShi. Industry and regional cross type of management system improves the response speed of enterprise to customer (market) and operational efficiency, which has laid an organization foundation for the advancement of the status in value chain and the rapid growth of revenue. This paper tentatively puts forward a system framework for the improvement of organizational responsiveness and the successful realization of cross-industry strategic transformation of local enterprises in emerging economies. The rapid change of market demand and technology integration in emerging economies leads to the gradual transformation of enterprise strategy, and the organizational structure response is dynamically adjusted. "Progressive strategic transformation and dynamic organizational structure response" mode has become an important feature of the enterprise's cross-industry transformation in emerging economies, which is helpful for understanding the phenomenon of cross industry upgrading in emerging economies, opening up "strategic transformation and organizational response to black boxes", and further enriches the cross-industrial upgrading theory and "Chandler's Structure follows strategy".