Abstract: Based on the limitation of existing research on the one-way impact of technological infrastructure investment on technological innovation, this paper focuses on the interaction effect between the two. Firstly, it analyzes the mechanism of the interaction effect on the theoretical level and then uses the panel data of 30 provinces in China from 2009 to 2015 to establish the simultaneous equations model to test the effect, the conclusions are as follows:(1)At the national level, there is a mutually reinforcing interactive effect between technology infrastructure investment and technological innovation. Technology infrastructure investment contributes significantly to the increase in technological innovation. Increasing technological innovation also helps to increase large technology infrastructure investment.(2)In terms of sub-regions, the contribution of over-investment in technology infrastructure in the eastern region has no significant effect on technological innovation. However, the central and western regions still show a mutually reinforcing interactive effect. There are differences in the impact of technology infrastructure investment on technological innovation among different regions, which are manifested in Central> Western> Eastern.