Abstract: Based on the amenities theory, this paper proposed an indicator system in five aspects including natural ecological environment, economic development level, social living environment, public service level, and science and education innovation environment, to evaluate a city’s attraction for Science and Technology Innovation Talents. This indicator system includes 5 categories of urban amenities and 49 indicators. By using entropy method and cluster analysis, the evaluation and comparative analysis of the comprehensive attractiveness of 33 major cities in China was carried out. The results show that big cities’ attractiveness such as Beijing, Shanghai, Guangzhou and Shenzhen are obviously higher than the cities in the central and western regions, and the scores of different cities in different dimensions are quite different, which means that different cities have different advantages and disadvantages. Major cities should combine their own characteristics and location conditions, then introduce corresponding policy systems to better attract Science and Technology Innovation Talents.