Abstract: The advantages of enterprises on sci-tech innovation board attracting investment are not the current profit status, but their profit prospects. This study provides suggestions for evaluation of Sci-Tech innovation board application and information disclosure content by studying the profitability of sci-tech innovation enterprises. In terms of methods, a total of 797 enterprises in a certain district of Shanghai in three years from 2015 to 2017 were taken as samples, and the growth of economic benefits was taken as the target. The influencing factors were studied from multiple aspects. The results show that the growth of economic benefits of enterprises is not directly related to the single variable of R&D investment, R&D output and economic benefits; On the other hand, a good circular mechanism between R&D investment, R&D output and economic benefit of an enterprise is an important feature that distinguishes the growth strength of an enterprise. Since the single indicator cannot provide a good assessment of long-term profitability, it is recommended to evaluate three aspects simultaneously: R&D investment, R&D output, and economic benefits. At the same time, it is suggested to cultivate the third-party markets for statistics and release other information, such as market share and exclusive technology etc. The information is very helpful for private investors to evaluate the enterprises on sci-tech innovation board but difficult to be achieved for them.