Abstract: In the face of the rapidly changing environment, how can enterprises internalize situational information and break through the rigid shackles of innovation ability to maintain continuous competitive advantage in the fierce market competition? The existing research lacks rigorous academic analysis. Based on the two-dimensional perspective of organizational practice, this paper deeply explores the construction mechanism of enterprise innovation ability, clarifies the root of enterprise building differentiated innovation ability, and opens the black box of endogenous reform of organizational practice. The research shows that :(1) The formation of context-based ostensive aspect of routines represents the organization's understanding and internalization process of situational differentiation, and is the root of enterprise's construction of differentiated innovation ability;(2) It is the key to promote enterprise innovation ability to break through the rigid shackles that organizational routines updates timely with the change of environment by virtue of its performative aspect and the ostensive aspect;(3) Environmental uncertainty provides situational requirements for the construction and development of innovation ability, and promotes the continuous upgrading of enterprise innovation ability from "imitation innovation ability" to "independent innovation ability" to "collaborative innovation ability".