Abstract: The network relationship established between new ventures and other actors in the entrepreneurial ecosystem can help them obtain key resources, develop entrepreneurial opportunities, promote their survival and development. However, the existing studies directly use the characteristics of general entrepreneurial network relationships to measure the network relationships built by new venture in the entrepreneurial ecosystem, seriously ignore the uniqueness of the network relationships in the entrepreneurial ecosystem, and it is more difficult to deeply reveal the role of the network relationships built by new venture in the entrepreneurial ecosystem. Therefore, based on the literature analysis of entrepreneurial ecosystem, the paper encodes and analyzes the qualitative interview materials, and deeply analyzes the connotation and dimension of the network relationship characteristic, compiles the initial measurement scale according to the standardized scale development program, and then carries out exploratory factor analysis, confirmatory factor analysis, reliability and validity test on the data obtained from the questionnaire survey. The results show that the network relationship of new venture in the entrepreneurial ecosystem has three characteristics: heterogeneity, multiplicity and variability. The final measurement scale has strong stability and reliability, which lays a theoretical foundation and provides the corresponding measurement tool for future studies.