Abstract: Abstract: For Large science and technology （construction） project organizations, knowledge management and learning organization complement each other, improve and support mutually. The learning organization is the carrier of knowledge management, and knowledge management is the core and source of the knowledge resources of the learning organization. Combining the knowledge production features of large project organizations, the paper studies on the coupling relationship between knowledge management and learning organization from the systematic and ecological perspectives, establishes the organizational culture, organizational strategy, organizational structure and organizational learning and knowledge flow four integrated key fields. And then according to the initial key fields, developing key fields, mature key and sustained improvement key four integrated optimization stages, the paper analyzes integrated optimization methods, builds a large science and technology （construction） project organization knowledge management and learning organization organically and symbiotically integrated optimization system body model.