Abstract: Knowledge evolution is the dynamic process to evaluate and create their own knowledge stock in order to meet the needs of organization knowledge application and the needs of innovation. This research takes high-tech enterprises as an example, put forward a basic analytical framework to study on the influence of knowledge mobilization to the knowledge evolution, design the knowledge mobilization as the independent variable, the knowledge evolution as a dependent variable, the knowledge base as a mediating variable and the knowledge hiding as a moderation variable. The results indicate that the knowledge mobilization has a positive impact on the knowledge evolution, the knowledge hiding plays a negative mediating role in knowledge mobilization and knowledge evolution, the knowledge base plays a moderation role between knowledge mobilization and knowledge evolution, that is strong knowledge based enterprises are easier to obtain the effect of knowledge mobilization, defuse the negative effects of knowledge hiding to the evolution of knowledge than weak knowledge based enterprises.