Abstract: In high-tech alliance innovation appropriability problems have become more prominent, so we have surveyed the alliance companies of high-tech industries in our country with 276 valid questionnaires, and researched the function mechanism of dual oriented innovation appropriability mechanisms on alliance performance. As a result, both formal and informal innovation appropriability mechanisms have a significant inversed U relationship with alliance performance, and there are better effects of dual oriented innovation appropriability mechanisms on alliance performance than single oriented innovation appropriability mechanisms. Moreover, knowledge mobility partially mediates the relationship between formal or informal innovation appropriability mechanisms and alliance performance. It shows that moderate innovation appropriability mechanisms may create an innovation atmosphere with trust, justice and transparence policy for valid knowledge mobility, and then improve alliance performance, and it also illustrates that there are different effects between different innovation appropriability mechanisms. Our results reveal the function pathway of innovation appropriability mechanisms in alliance, which provide a guide for the high-tech alliance companies to coordinate different innovation appropriability mechanisms.