Abstract: Firstly, based on the two image duality theory, the technology hatching innovation system is divided into three parts: resource subsystem, incubator innovation performance subsystem and innovation customer performance subsystem. Then, based on the coordinated coupling degree model, the degree of synergistic development of the 31 provincial technology incubators in 2009-2016 years was measured, and the spatial and temporal characteristics were revealed. Thirdly, the Dynamic Spatial Durbin Model is established to explore the influence factors of regional innovation performance and its spatial spillover effect. At last, taking government support as threshold variable, establishing threshold regression model, and conduct a nonlinear test on the role of government support in the process of developing technology on regional innovation. The results showed that: (1) Synergy degree of technology incubating industry and government support have a significant positive effect on regional innovation, regional innovation was affected positively by itself and was affected negatively by the performance of adjacent areas .(2) There are the “direct effects” and “spillover effects” of Synergistic development of technology incubating industry on regional innovation. (3) As for the Synergy degree of technology incubating industry, its long-term effect is less than the short-term effect. In the short term, the spillover effect is greater than the direct effect; in the long term, the direct effect is greater than the spillover effect. (4) Government support negatively regulate the relationship between technology incubating industry cooperation and regional innovation. (5) In the process of collaborative development of technology incubating industry, government support has non-linear effects, and the negative regulation will be weaken. Some industrial policies and suggestions are put forward according to the above conclusions.