Abstract: In order to explore the influence between regional soft environment factors and high-tech enterprise growth and differences in different regions，based on resource dependence theory，selected 30 China （city，district）provinces panel data from 2007 to 2013 used spatial lag model to test the relationship between five regional environment factors（technology environment，market environment，financial environment，government environment，legal environment）and high-tech enterprise growth. Results showed that: From national perspective，technology environment and government environment were major factors affected high-tech enterprise growth，financial environment and legal environment had limited impact on high-tech enterprise growth，market environment had a negative impact on high-tech enterprise growth. From region perspective，the influence between five regional soft environment factors and high-tech enterprise growth had a significant difference. Among them，technology environment had a positive impact on high-tech enterprise growth in the Eastern，Central，Western and Northeast region，financial environment in these four regions had no effect，market environment had a negative impact in the Central and Western region，government environment had a positive impact in the Central and Western region，legal environment had a negative impact in the Northeast region.