Abstract: Maker space is an important carrier to support the innovation and entrepreneurship of new venture, the entrepreneurial environment of maker space is an important factor affecting the new venture performance. This study explores the impact mechanism of maker space entrepreneurial environment on the new venture performance from the perspective of entrepreneurial ecosystem. An empirical study was conducted by taking 1902 maker spaces that have been included into the management service system of national science and technology business incubator as the research sample, and R language tool is used for verification. The results show that the platform cooperation environment, entrepreneurial training environment and financial support environment of maker space have a significant positive impact on the capital performance, talent performance and innovation performance of new venture. And the technology supply environment has a significant positive impact on the capital performance of new venture. The type of entered entrepreneurial team plays a moderating role in the impact of entrepreneurial environment on the new venture performance. The type of executive turnover has positive moderating effect between the technology supply environment and the capital performance of new venture. The type of scientific and technical personnel has a negative moderating effect between the platform cooperation environment and the capital performance of new venture. The type of returned from abroad has a positive moderating effect between the entrepreneurial training environment and the capital performance and innovation performance of new venture. The type of college students has negative a moderating effect between the financial support environment and the capital performance and innovation performance of new venture. The conclusions provide practical guidance for improving the new venture performance, helping entrepreneurs choose the maker space and the construction of the maker space.